Mouse HSPA5/BIP Recombinant Protein (N-His) (MY489012)

Name: Mouse HSPA5/BIP Recombinant Protein (N-His)
Brand: abinScience
SKU: MY489012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MY489012
Description	Recombinant Mouse HSPA5/BIP Protein, N-His (MY489012) expressed in E. coli, spanning Met1-Leu654. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P20029
Protein length	Met1-Leu654
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	74.49 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Endoplasmic reticulum chaperone BiP, 3.6.4.10, 78 kDa glucose-regulated protein, GRP-78, Binding-immunoglobulin protein, BiP, Heat shock protein 70 family protein 5, HSP70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, Hspa5, Grp78
Background	Endoplasmic reticulum chaperone BiP (HSPA5/GRP78) is a ~72 kDa protein. Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the EIF2AK3/PERK and ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Also binds and inactivates EIF2AK3/PERK in unstressed cells. 1. Cunnea, PM. et al. (2003) The Journal of biological chemistry 278, 1059-66. PMID: 12411443 2. Meunier, L. et al. (2002) Molecular biology of the cell 13, 4456-69. PMID: 12475965 3. Bertolotti, A. et al. (2000) Nature cell biology 2, 326-32. PMID: 10854322
Note	For research use only.

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SDS-PAGE for Recombinant Mouse HSPA5/BIP Protein, N-His

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Datasheet

Mouse HSPA5/BIP Recombinant Protein (N-His).pdf

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Mouse HSPA5/BIP Recombinant Protein (N-His) (MY489012)

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