Mouse IL-13 Recombinant Protein (C-Fc) (MW632011)

Name: Mouse IL-13 Recombinant Protein (C-Fc)
Brand: abinScience
SKU: MW632011
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	MW632011
Description	Recombinant Mouse IL13 Protein, C-Fc (MW632011) expressed in Mammalian Cells, spanning Leu17-Phe131. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	P20109
Protein length	Leu17-Phe131
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	40.68 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Interleukin-13, IL-13, T-cell activation protein P600, Il13, Il-13
Background	Interleukin-13 (IL13) is a ~14 kDa protein. Cytokine that plays important roles in allergic inflammation and immune response to parasite infection. Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells. Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1. Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF through a mechanism that partially involves suppression of NF-kappa-B. 1. Finkelman, FD. et al. (2004) Immunological reviews 201, 139-55. PMID: 15361238 2. Zhang, M. et al. (2021) Journal of immunology (Baltimore, Md. : 1950) 207, 3070-3080. PMID: 34789557 3. Mayer, JU. et al. (2021) Nature immunology 22, 1538-1550. PMID: 34795444 4. Takeda, K. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 157, 3220-2. PMID: 8871614 5. Duffen, J. et al. (2018) Journal of immunology (Baltimore, Md. : 1950) 200, 1347-1359. PMID: 29305434
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Mouse IL13 Protein, C-Fc
Bioactivity

Detects Mouse IL13 Antibody (BAK209B11) in indirect ELISAs.

References

Datasheet

Mouse IL-13 Recombinant Protein (C-Fc).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Mouse IL-13 Recombinant Protein (C-Fc) (MW632011)

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