Mouse JAM3 Recombinant Protein (N-His) (MP669012)

Name: Mouse JAM3 Recombinant Protein (N-His)
Brand: abinScience
SKU: MP669012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MP669012
Description	Recombinant Mouse JAM3 Protein, N-His (MP669012) expressed in E. coli, spanning Glu30-Asp239. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9D8B7
Protein length	Glu30-Asp239
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.92 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	JAM-3, sJAM-C, JAM-C, Jam3, Junctional adhesion molecule 3, Junctional adhesion molecule C, JAM-2
Background	Junctional adhesion molecule C (JAM3) is a ~34 kDa protein. Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM2 to regulate different cellular processes. Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3. Plays a central role in leukocytes extravasation by facilitating transmigration through the endothelium. Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation. 1. Gliki, G. et al. (2004) Nature 431, 320-4. PMID: 15372036 2. Lamagna, C. et al. (2005) Molecular biology of the cell 16, 4992-5003. PMID: 16093349 3. Arcangeli, ML. et al. (2014) Stem cells (Dayton, Ohio) 32, 1043-54. PMID: 24357068 4. Ludwig, RJ. et al. (2005) The Journal of investigative dermatology 125, 969-76. PMID: 16297198 5. Lamagna, C. et al. (2005) Cancer research 65, 5703-10. PMID: 15994945
Note	For research use only.

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SDS-PAGE for Recombinant Mouse JAM3 Protein, N-His

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Datasheet

Mouse JAM3 Recombinant Protein (N-His).pdf

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Mouse JAM3 Recombinant Protein (N-His) (MP669012)

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