Mouse KIRREL1 Recombinant Protein (N-His) (MA258012)

Name: Mouse KIRREL1 Recombinant Protein (N-His)
Brand: abinScience
SKU: MA258012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MA258012
Description	Recombinant Mouse KIRREL1 Protein, N-His (MA258012) expressed in E. coli, spanning Thr51-Glu523. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q80W68
Protein length	Thr51-Glu523
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	54.17 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	NEPH1, KIRREL1, Kin of IRRE-like protein 1, KIRREL, Nephrin-like protein 1, Kin of irregular chiasm-like protein 1
Background	Kin of IRRE-like protein 1 (KIRREL1) is a ~87 kDa protein. Required for proper function of the glomerular filtration barrier. It is involved in the maintenance of a stable podocyte architecture with interdigitating foot processes connected by specialized cell-cell junctions, known as the slit diaphragm. Is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1. 1. Donoviel, DB. et al. (2001) Molecular and cellular biology 21, 4829-36. PMID: 11416156
Note	For research use only.

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SDS-PAGE for Recombinant Mouse KIRREL1 Protein, N-His

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Datasheet

Mouse KIRREL1 Recombinant Protein (N-His).pdf

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Mouse KIRREL1 Recombinant Protein (N-His) (MA258012)

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