Mouse OSTN Recombinant Protein (N-GST & C-His) (MX160012)

Name: Mouse OSTN Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: MX160012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MX160012
Description	Recombinant Mouse OSTN Protein, N-GST & C-His (MX160012) expressed in E. coli, spanning Phe26-Gly130. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P61364
Protein length	Phe26-Gly130
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	39.79 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	OSTN, Osteocrin, Musclin
Background	Osteocrin (OSTN) is a ~14 kDa protein. Hormone that acts as a ligand for natriuretic peptide receptor NPR3/NPR-C and promotes bone growth and physical endurance in muscle. Acts as a regulator of osteoblast differentiation and bone growth by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production. Required to enhance physical endurance: induced following physical exercise in muscle and promotes cGMP production, probably by interacting with NPR3/NPR-C. May act as an autocrine and paracrine factor linked to glucose metabolism in skeletal muscle. 1. Thomas, G. et al. (2003) The Journal of biological chemistry 278, 50563-71. PMID: 14523025 2. Moffatt, P. et al. (2007) The Journal of biological chemistry 282, 36454-62. PMID: 17951249 3. Subbotina, E. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 16042-7. PMID: 26668395 4. Nishizawa, H. et al. (2004) The Journal of biological chemistry 279, 19391-5. PMID: 15044443
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Mouse OSTN Protein, N-GST & C-His

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Datasheet

Mouse OSTN Recombinant Protein (N-GST & C-His).pdf

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C₁ × V₁ = C₂ × V₂

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Mouse OSTN Recombinant Protein (N-GST & C-His) (MX160012)

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