Mouse PGLYRP1 Recombinant Protein (N-His) (MF951012)

Name: Mouse PGLYRP1 Recombinant Protein (N-His)
Brand: abinScience
SKU: MF951012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MF951012
Description	Recombinant Mouse PGLYRP1 Protein, N-His (MF951012) expressed in E. coli, spanning Val41-Glu182. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O88593
Protein length	Val41-Glu182
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.50 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Pglyrp, Peptidoglycan recognition protein 1, Pgrps, Cytokine tag7, Peptidoglycan recognition protein short, PGRP-S, Pglyrp1, Tag7, Pgrp
Background	Peptidoglycan recognition protein 1 (PGLYRP1) is a ~20 kDa protein. Innate immunity protein that plays several important functions in antimicrobial and antitumor defense systems. Acts as a pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria and thus provides bactericidal activity. Forms an equimolar complex with heat shock protein HSPA1A and induces programmed cell death through apoptosis and necroptosis in tumor cell lines by activating the TNFR1 receptor on the target cell membrane. In addition, acts in complex with the Ca(2+)-binding protein S100A4 as a chemoattractant able to induce lymphocyte movement. Mechanistically, this complex acts as a ligand of the chemotactic receptors CCR5 and CXCR3 which are present on the cells of the immune system. 1. Dziarski, R. et al. (2003) Blood 102, 689-97. PMID: 12649138 2. Kiselev, SL. et al. (1998) The Journal of biological chemistry 273, 18633-9. PMID: 9660837 3. Kang, D. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 10078-82. PMID: 9707603 4. Sashchenko, LP. et al. (2004) The Journal of biological chemistry 279, 2117-24. PMID: 14585845
Note	For research use only.

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SDS-PAGE for Recombinant Mouse PGLYRP1 Protein, N-His

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Datasheet

Mouse PGLYRP1 Recombinant Protein (N-His).pdf

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Mouse PGLYRP1 Recombinant Protein (N-His) (MF951012)

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