Mouse PPP2R1A Recombinant Protein (C-His) (MB105012)

Name: Mouse PPP2R1A Recombinant Protein (C-His)
Brand: abinScience
SKU: MB105012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MB105012
Description	Recombinant Mouse PPP2R1A Protein, C-His (MB105012) expressed in E. coli, spanning Met1-Ala589. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q76MZ3
Protein length	Met1-Ala589
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	66.38 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, Ppp2r1a
Background	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A) is a ~65 kDa protein. The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis. Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway. Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability. 1. Nanahoshi, M. et al. (1999) FEBS letters 446, 108-12. PMID: 10100624 2. Apostolidis, SA. et al. (2016) Nature immunology 17, 556-64. PMID: 26974206 3. Chou, WC. et al. (2021) Nature 591, 300-305. PMID: 33505023
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Mouse PPP2R1A Protein, C-His

References

MB105012-SDS-PAGE-1.jpg

Datasheet

Mouse PPP2R1A Recombinant Protein (C-His).pdf

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Mouse PPP2R1A Recombinant Protein (C-His) (MB105012)

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