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Mouse RNASE4 Recombinant Protein (C-His) (MW563011)

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Overview
Catalog No.MW563011
Description
Recombinant Mouse RNASE4 Protein, C-His (MW563011) expressed in Yeast Cells, spanning Gln30-Arg148. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemYeast cells
AccessionQ9JJH1
Protein lengthGln30-Arg148
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMus musculus (Mouse)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 15.17 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEC:3.1.27.-, RNASE4, RNS4, RNase 4, Ribonuclease 4
Background

Ribonuclease 4 is a ~17 kDa protein. Cleaves preferentially after uridine bases. Has antimicrobial activity against uropathogenic E.coli (UPEC). Probably contributes to urinary tract sterility.

1. Murtha, MJ. et al. (2018) The Journal of clinical investigation 128, 5634-5646. PMID: 30418175
Note For research use only
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References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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