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Mouse SLC1A2 Recombinant Protein (N-His) (MW629012)

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Overview
Catalog No.MW629012
Description
Recombinant Mouse SLC1A2 Protein, N-His (MW629012) expressed in E. coli, spanning Tyr493-Lys572. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionP43006
Protein lengthTyr493-Lys572
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMus musculus (Mouse)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 11.51 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEAAT2, Excitatory amino acid transporter 2, GLT1, Glutamate/aspartate transporter II, SLC1A2, Sodium-dependent glutamate/aspartate transporter 2, Solute carrier family 1 member 2
Background

Excitatory amino acid transporter 2 is a ~62 kDa protein. Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate.

1. Sutherland, ML. et al. (1995) Gene 162, 271-4. PMID: 7557442
2. Kirschner, MA. et al. (1994) Genomics 24, 218-24. PMID: 7698742
3. Utsunomiya-Tate, N. et al. (1997) FEBS letters 416, 312-6. PMID: 9373176
4. Tanaka, K. et al. (1997) Science (New York, N.Y.) 276, 1699-702. PMID: 9180080
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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