Mouse SOX9 Recombinant Protein (N-His) (MW480012)

Name: Mouse SOX9 Recombinant Protein (N-His)
Brand: abinScience
SKU: MW480012
Price: 211 USD
Availability: InStock

Datasheet Share product Copy info

Notice · Catalog MW480012 has been discontinued.

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	MW480012
Description	Recombinant Mouse SOX9 Protein, N-His (MW480012) expressed in E. coli, spanning Met1-Lys151. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q04887
Protein length	Met1-Lys151
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Transcription factor SOX-9, SOX9
Background	Transcription factor SOX-9 (SOX9) is a ~56 kDa protein. Transcription factor that plays a key role in chondrocytes differentiation and skeletal development. Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6. Also binds to some promoter regions. Plays a central role in successive steps of chondrocyte differentiation. Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes. 1. Bi, W. et al. (1999) Nature genetics 22, 85-9. PMID: 10319868 2. Bi, W. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 6698-703. PMID: 11371614 3. Akiyama, H. et al. (2002) Genes & development 16, 2813-28. PMID: 12414734 4. Akiyama, H. et al. (2004) Genes & development 18, 1072-87. PMID: 15132997 5. Hargus, G. et al. (2008) The International journal of developmental biology 52, 323-32. PMID: 18415932 6. Takigawa, Y. et al. (2010) Journal of cell science 123, 3780-8. PMID: 20940257 7. Egunsola, AT. et al. (2017) The Journal of clinical investigation 127, 1475-1484. PMID: 28263186 8. Huang, W. et al. (2000) Molecular and cellular biology 20, 4149-58. PMID: 10805756 9. Akiyama, H. et al. (2005) Matrix biology : journal of the International Society for Matrix Biology 23, 499-505. PMID: 15694126
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Mouse SOX9 Protein, N-His

References

Datasheet

Mouse SOX9 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Mouse SOX9 Recombinant Protein (N-His) (MW480012)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us