ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Mus musculus (Mouse)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
10.42 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
mRNA decay activator protein ZFP36, Growth factor-inducible nuclear protein NUP475, TPA-induced sequence 11, Tristetraprolin, Zinc finger protein 36, Zfp-36, Zfp36, Nup475, Tis11, Tis11a, Ttp
Background
mRNA decay activator protein ZFP36 is a ~33 kDa protein. Zinc-finger RNA-binding protein that destabilizes numerous cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as an 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1, and hence promotes ARE-mediated mRNA deadenylation. Also functions by recruiting components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs. Self-regulates by destabilizing its own mRNA.
1. Lai, WS. et al. (1999) Molecular and cellular biology 19, 4311-23. PMID: 10330172
2. Carballo, E. et al. (2000) Blood 95, 1891-9. PMID: 10706852
3. Stoecklin, G. et al. (2000) Molecular and cellular biology 20, 3753-63. PMID: 10805719
4. Stoecklin, G. et al. (2004) The EMBO journal 23, 1313-24. PMID: 15014438
5. Tchen, CR. et al. (2004) The Journal of biological chemistry 279, 32393-400. PMID: 15187092
6. Ogilvie, RL. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 174, 953-61. PMID: 15634918
7. Lai, WS. et al. (2006) Molecular and cellular biology 26, 9196-208. PMID: 17030620
8. Horner, TJ. et al. (2009) Molecular and cellular biology 29, 1999-2010. PMID: 19188452
9. Marchese, FP. et al. (2010) The Journal of biological chemistry 285, 27590-600. PMID: 20595389
10. Clement, SL. et al. (2011) Molecular and cellular biology 31, 256-66. PMID: 21078877
Note
For research use only
Images
SDS-PAGE
SDS-PAGE for Recombinant Mouse ZFP36 Protein, N-His
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