Mouse ZFP36L2 Recombinant Protein (N-GST & C-His) (MC122012)

Name: Mouse ZFP36L2 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: MC122012
Price: 211 USD
Availability: InStock

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Catalog No.	MC122012
Description	Recombinant Mouse ZFP36L2 Protein, N-GST & C-His (MC122012) expressed in E. coli, spanning Ser127-Pro227. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P23949
Protein length	Ser127-Pro227
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	39.90 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	mRNA decay activator protein ZFP36L2, Butyrate response factor 2, TPA-induced sequence 11d, Zinc finger protein 36 C3H1 type-like 2, ZFP36-like 2, Zfp36l2, Tis11d
Background	mRNA decay activator protein ZFP36L2 is a ~50 kDa protein. Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis. Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery. Functions by recruiting the CCR4-NOT deadenylase complex and probably other components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes. Binds to 3'-UTR ARE of numerous mRNAs. Promotes ARE-containing mRNA decay of the low-density lipoprotein (LDL) receptor (LDLR) mRNA in response to phorbol 12-myristate 13-acetate (PMA) treatment in a p38 MAPK-dependent manner. 1. Ramos, SB. (2012) The Journal of biological chemistry 287, 13116-27. PMID: 22367205 2. Lin, NY. et al. (2012) International journal of biological sciences 8, 761-77. PMID: 22701344 3. Ball, CB. et al. (2014) PloS one 9, e97324. PMID: 24830504 4. Cargnin, F. et al. (2014) The Journal of neuroscience : the official journal of the Society for Neuroscience 34, 16650-61. PMID: 25505318 5. Galloway, A. et al. (2016) Science (New York, N.Y.) 352, 453-9. PMID: 27102483
Note	For research use only

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Mouse ZFP36L2 Recombinant Protein (N-GST & C-His).pdf

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Mouse ZFP36L2 Recombinant Protein (N-GST & C-His) (MC122012)

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