MRV-3 Sigma3/S4 Recombinant Protein (N-His) (VK094022)

Name: MRV-3 Sigma3/S4 Recombinant Protein (N-His)
Brand: abinScience
SKU: VK094022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK094022
Description	Recombinant MRV-3 Sigma3/S4 Protein, N-His (VK094022) expressed in E. coli, spanning Met1-Gly365. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P03527
Protein length	Met1-Gly365
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mammalian orthoreovirus 3 (MRV3)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	43.28 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	S4
Background	Outer capsid protein sigma-3 is a ~41 kDa protein. Outer layer capsid protein that stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding. During virus assembly, the inner layer proteins lambda-1 and the turret protein lambda-2 form the star core, followed by the stepwise attachment of sigma-2 that completes the core and mu1/sigma3 that complete the outer layer. Binds to the host neuronal receptor RTN4R for viral entry into host neural cells. 1. Yue, Z. et al. (1997) Virology 234, 364-71. PMID: 9268168 2. Liu, X. et al. (2024) Nature communications 15, 10638. PMID: 39639006 3. Sutherland, DM. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2219404120. PMID: 37276413
Note	For research use only

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SDS-PAGE for Recombinant MRV-3 Sigma3/S4 Protein, N-His

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Datasheet

MRV-3 Sigma3/S4 Recombinant Protein (N-His).pdf

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MRV-3 Sigma3/S4 Recombinant Protein (N-His) (VK094022)

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