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Mycoplasma pneumoniae Wheel complex P24 Recombinant Protein (N-His) (JN180022)

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Overview
Catalog No.JN180022
Description
Recombinant Mycoplasma pneumoniae Wheel complex protein P24, N-His (JN180022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionP75469
Protein lengthMet1-Lys218
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMycoplasma pneumoniae
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesWheel complex protein P24
Background

Wheel complex protein P24 is a ~24 kDa protein. Component of the wheel complex, part of the cytoskeleton-like structure in the attachment organelle which stabilizes the shape of the wall-less Mycoplasma. Might play a role in coordinating gliding initiation with P41 (Probable). P24 alone does not restore gliding motility or frequency to a P41-P24 disruption, but it is required for full wild-type gliding frequency and for correct initation of attachment organelle formation. The attachment organelle is involved in gliding cell motility and adhesion to host cells.

1. Nakane, D. et al. (2015) PLoS pathogens 11, e1005299. PMID: 26633540
2. Kenri, T. et al. (2004) Journal of bacteriology 186, 6944-55. PMID: 15466048
3. Hasselbring, BM. et al. (2007) Journal of bacteriology 189, 7442-9. PMID: 17693502
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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