

| Catalog No. | JN180012 |
|---|---|
| Description |
Recombinant Mycoplasma pneumoniae Wheel complex protein TopJ, N-His (JN180012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Expression system | E. coli |
| Accession | P75354 |
| Protein length | Lys678-His910 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Mycoplasma pneumoniae |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | Wheel complex protein TopJ, topj |
| Background | Wheel complex protein TopJ is a ~100 kDa protein. Component of the wheel complex, part of the cytoskeleton-like structure in the attachment organelle which stabilizes the shape of the wall-less Mycoplasma. Required for correct assembly of adhesin P1 and positioning/maturation of attachment organelle; may function as a co-chaperone with DnaK. The attachment organelle is involved in gliding cell motility and adhesion to host cells. 1. Cloward, JM. et al. (2010) Molecular microbiology 77, 158-69. PMID: 20487283 2. Nakane, D. et al. (2015) PLoS pathogens 11, e1005299. PMID: 26633540 3. Cloward, JM. et al. (2011) Molecular microbiology 81, 528-39. PMID: 21631602 |
| Note | For research use only |
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