Porphyromonas gingivalis fimA Recombinant Protein (N-His) (JN094012)

Name: Porphyromonas gingivalis fimA Recombinant Protein (N-His)
Brand: abinScience
SKU: JN094012
Price: 211 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	JN094012
Description	Recombinant Porphyromonas gingivalis fimA Protein, N-His (JN094012) expressed in E. coli, spanning Asn20-Trp383. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	B2RH54
Protein length	Asn20-Trp383
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Porphyromonas gingivalis
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	41.62 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Major fimbrium subunit FimA type-1, Fimbrillin, Fimbrilin, Major fimbrial subunit protein type I, fimA
Background	Major fimbrium subunit FimA type-1 is a ~41 kDa protein. Structural subunit of the major fimbriae. These long, filamentous pili are attached to the cell surface; they mediate biofilm formation, adhesion onto host cells and onto other bacteria that are part of the oral microbiome. They play an important role in the invasion of periodontal tissues. Fimbriae and their constituents are major virulence factors. FimA proteins from different strains have highly divergent sequences, and this has been used for classification. 1. Umemoto, T. et al. (2003) Journal of periodontology 74, 119-22. PMID: 12593606 2. Shoji, M. et al. (2004) Molecular microbiology 52, 1513-25. PMID: 15165251 3. Nishiyama, SI. et al. (2007) Microbiology (Reading, England) 153, 1916-1925. PMID: 17526848 4. Wang, M. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 179, 2349-58. PMID: 17675496 5. Nagano, K. et al. (2010) Journal of dental research 89, 903-8. PMID: 20530728 6. Xu, Q. et al. (2016) Cell 165, 690-703. PMID: 27062925 7. Kadowaki, T. et al. (1998) The Journal of biological chemistry 273, 29072-6. PMID: 9786913 8. Nagano, K. et al. (2013) Molecular oral microbiology 28, 392-403. PMID: 23809984
Note	For research use only.

Images

Bioactivity

Detected by Anti-P.gingivalis fimA/Fimbrilin Antibody (123-123-10) (Cat No.: JN847013) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Porphyromonas gingivalis fimA Recombinant Protein (N-His)

References

Datasheet

Porphyromonas gingivalis fimA Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Porphyromonas gingivalis fimA Recombinant Protein (N-His) (JN094012)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us