Rat SCNN1A/SCNN1 Recombinant Protein (N-His) (RW302012)

Name: Rat SCNN1A/SCNN1 Recombinant Protein (N-His)
Brand: abinScience
SKU: RW302012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	RW302012
Description	Recombinant Rat SCNN1A/SCNN1 Protein, N-His (RW302012) expressed in E. coli, spanning Glu132-Asp391. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P37089
Protein length	Glu132-Asp391
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Rattus norvegicus (Rat)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	32.25 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial Na(+) channel subunit alpha, Alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, Scnn1a, Renac
Background	Epithelial sodium channel subunit alpha (SCNN1A/SCNN1) is a ~78 kDa protein. This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys. Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance. 1. Lingueglia, E. et al. (1994) The Journal of biological chemistry 269, 13736-9. PMID: 8188647 2. Gründer, S. et al. (1997) The EMBO journal 16, 899-907. PMID: 9118951 3. Lingueglia, E. et al. (1993) FEBS letters 318, 95-9. PMID: 8382172
Note	For research use only.

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SDS-PAGE for Recombinant Rat SCNN1A/SCNN1 Protein, N-His

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Datasheet

Rat SCNN1A/SCNN1 Recombinant Protein (N-His).pdf

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Rat SCNN1A/SCNN1 Recombinant Protein (N-His) (RW302012)

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