Rat SLC22A6 Recombinant Protein (N-His) (RS924012)

Name: Rat SLC22A6 Recombinant Protein (N-His)
Brand: abinScience
SKU: RS924012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	RS924012
Description	Recombinant Rat SLC22A6 Protein, N-His (RS924012) expressed in E. coli, spanning Met31-Gln135. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O35956
Protein length	Met31-Gln135
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Rattus norvegicus (Rat)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	13.86 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Solute carrier family 22 member 6, Organic anion transporter 1, rOAT1, renal organic anion transporter 1, rROAT1, Slc22a6, Oat1
Background	Solute carrier family 22 member 6 is a ~60 kDa protein. Secondary active transporter that functions as a Na(+)-independent organic anion (OA)/dicarboxylate antiporter where the uptake of one molecule of OA into the cell is coupled with an efflux of one molecule of intracellular dicarboxylate such as alpha-ketoglutarate or glutarate. Mediates the uptake of OA across the basolateral side of proximal tubule epithelial cells, thereby contributing to the renal elimination of endogenous OA from the systemic circulation into the urine. Function as a biopterin transporters involved in the uptake and the secretion of coenzymes tetrahydrobiopterin (BH4) dihydrobiopterin (BH2) and sepiapterin to urine, thereby determining baseline levels of blood biopterins. Transports prostaglandin E2 (PGE2) and prostaglandin F2-alpha (PGF2-alpha) and may contribute to their renal excretion. Also mediates the uptake of cyclic nucleotides such as cAMP and cGMP. 1. Deguchi, T. et al. (2004) Kidney international 65, 162-74. PMID: 14675047 2. Uwai, Y. et al. (2013) Bioscience, biotechnology, and biochemistry 77, 1517-21. PMID: 23832370 3. Sekine, T. et al. (1997) The Journal of biological chemistry 272, 18526-9. PMID: 9228014 4. Sweet, DH. et al. (1997) The Journal of biological chemistry 272, 30088-95. PMID: 9374486
Note	For research use only

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SDS-PAGE for Recombinant Rat SLC22A6 Protein, N-His

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Datasheet

Rat SLC22A6 Recombinant Protein (N-His).pdf

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Formula

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Rat SLC22A6 Recombinant Protein (N-His) (RS924012)

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