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Sapporo virus NS4 Recombinant Protein (N-His) (VK020022)

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Sapporo virus NS4 Recombinant Protein (N-His)

Sapporo virus NS4 Recombinant Protein (N-His)

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1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	VK020022
Description	Recombinant Sapporo virus NS4 Protein, N-His (VK020022) expressed in E. coli, spanning Ala666-Glu939. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q672I1
Protein length	Ala666-Glu939
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Sapporo virus
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90%
Predicted molecular weight	32.77 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Protein p32
Background	Genome polyprotein (NS4) is a ~251 kDa protein. Together with NTPase and NS4, initiates the formation of the replication complex. Induces the proliferation of the host smooth ER membranes forming long tubular structures. These remodeled membranes probably form the viral factories that contain the replication complex.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Sapporo virus NS4 Protein, N-His

References

Datasheet

Sapporo virus NS4 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

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Stock Solution

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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Sapporo virus NS4 Recombinant Protein (N-His)