Staphylococcus aureus srtA/Sortase A Recombinant Protein (C-His) (JN051022)

Name: Staphylococcus aureus srtA/Sortase A Recombinant Protein (C-His)
Brand: abinScience
SKU: JN051022
Price: 211 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD): --

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	JN051022
Description	Recombinant Staphylococcus aureus Sortase A/srtA Protein, C-His (JN051022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	WP_084930864.1
Protein length	Gln23-Lys169
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Staphylococcus aureus
Nature	Recombinant
Endotoxin level	Remove endotoxin and sterilization is required for cell assay.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	17.85 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Sortase A (srtA) is a ~23 kDa protein. Transpeptidase that anchors surface proteins to the cell wall. Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall. This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues. Utilizes lipid II as the peptidoglycan substrate for the sorting reaction. 1. Mazmanian, SK. et al. (1999) Science (New York, N.Y.) 285, 760-3. PMID: 10427003 2. Ton-That, H. et al. (1999) The Journal of biological chemistry 274, 24316-20. PMID: 10446208 3. Ton-That, H. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 12424-9. PMID: 10535938 4. Ton-That, H. et al. (2002) The Journal of biological chemistry 277, 7447-52. PMID: 11714722 5. Kruger, RG. et al. (2004) Biochemistry 43, 1541-51. PMID: 14769030 6. Marraffini, LA. et al. (2004) The Journal of biological chemistry 279, 37763-70. PMID: 15247224
Note	For research use only

Images

SDS-PAGE

SDS-PAGE for Staphylococcus aureus Sortase A/srtA Recombinant Protein (C-His)
Activity

Sortase A (SrtA) could cleave and catalyze Sortase Substrate III, Abz/DNP. The Sortase A activity is tested by the cleavage of substrate and detecting at Ex/Em=320 nm/420 nm.

References

Datasheet

Staphylococcus aureus srtA/Sortase A Recombinant Protein (C-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Staphylococcus aureus srtA/Sortase A Recombinant Protein (C-His) (JN051022)

Overview

Images

References

Datasheet

SDS