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Streptococcus pneumoniae psaA Recombinant Protein (N-His) (JN184042)

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Overview
Catalog No.JN184042
Description
Recombinant Streptococcus pneumoniae psaA Protein, N-His (JN184042) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionP0A4G2
Protein lengthLys34-Lys309
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesStreptococcus pneumoniae
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesManganese ABC transporter substrate-binding lipoprotein PsaA, Pneumococcal surface adhesin A, psaA
Background

Manganese ABC transporter substrate-binding lipoprotein PsaA is a ~34 kDa protein. Part of the ATP-binding cassette (ABC) transport system PsaABC involved in manganese import. Binds manganese with high affinity and specificity and delivers it to the membrane permease for translocation into the cytoplasm. Also acts as an adhesin which is involved on adherence to extracellular matrix. It is an important factor in pathogenesis and infection.

1. McDevitt, CA. et al. (2011) PLoS pathogens 7, e1002357. PMID: 22072971
2. Dintilhac, A. et al. (1997) Molecular microbiology 25, 727-39. PMID: 9379902
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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