Vaccinia virus/VACV F13L Recombinant Protein (C-His) (VK455022)

Name: Vaccinia virus/VACV F13L Recombinant Protein (C-His)
Brand: abinScience
SKU: VK455022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK455022
Brand	abinScience
Description	Recombinant Vaccinia virus/VACV F13L Protein, C-His (VK455022) expressed in E. coli, spanning Met1-Ile372. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P04021
Protein length	Met1-Ile372
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Vaccinia virus (VACV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	43.97 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	37 kDa protein, EC:3.1.1.-, EC:3.1.4.4, Envelope phospholipase F13, Envelope phospholipase OPG057, OPG057, Palmitoylated EV membrane protein, p37K
Background	Envelope phospholipase OPG057 (F13L) is a ~41 kDa protein. Major envelope protein that plays a role in the biogenesis of the viral double membrane and in egress of virus from the host cell. Produces the wrapped form of virus that is required for cell-to-cell spread. Acts as a lipase with broad specificity including phospholipase C, phospholipase A, and triacylglycerol lipase activities. 1. Honeychurch, KM. et al. (2007) Journal of virology 81, 7310-5. PMID: 17475658 2. Sivan, G. et al. (2016) Journal of virology 90, 8891-905. PMID: 27466413 3. Grosenbach, DW. et al. (1997) The Journal of biological chemistry 272, 1956-64. PMID: 8999886 4. Bryk, P. et al. (2018) Journal of virology 92. PMID: 29540596 5. Baek, SH. et al. (1997) The Journal of biological chemistry 272, 32042-9. PMID: 9405398
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Vaccinia virus/VACV F13L Protein, C-His

References

Datasheet

Vaccinia virus/VACV F13L Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

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V₂ (Working Vol.)

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Vaccinia virus/VACV F13L Recombinant Protein (C-His) (VK455022)

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