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WEEV Spike glycoprotein E2 Recombinant Protein (N-His) (VK634032)

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WEEV Spike glycoprotein E2 Recombinant Protein (N-His)

WEEV Spike glycoprotein E2 Recombinant Protein (N-His)

WEEV Spike glycoprotein E2 Recombinant Protein (N-His)

WEEV Spike glycoprotein E2 Recombinant Protein (N-His)

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	VK634032
Description	Recombinant WEEV Spike glycoprotein E2 Protein, N-His (VK634032) expressed in E. coli, spanning Ser320-Val687. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P13897
Protein length	Ser320-Val687
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Western equine encephalitis virus (WEEV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Structural polyprotein, p130, Capsid protein, 3.4.21.90, Coat protein, C, Precursor of protein E3/E2, p62, pE2, Assembly protein E3, Spike glycoprotein E2, E2 envelope glycoprotein, 6K protein, Spike glycoprotein E1, E1 envelope glycoprotein
Background	Structural polyprotein (Spike glycoprotein E2) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant WEEV Spike glycoprotein E2 Protein, N-His
Western blot

Western blot analysis was performed using anti-E2 polyclonal antibody (Catalog No: VK634034) at 1ug/mL on recombinant VEEV Spike glycoprotein E1.

References

Datasheet

WEEV Spike glycoprotein E2 Recombinant Protein (N-His).pdf

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Formula

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WEEV Spike glycoprotein E2 Recombinant Protein (N-His)