Anti-ChoP/Phosphorylcholine Reference Antibody (ATH3G10, RUO) (HP719016) is a human monoclonal antibody detecting ChoP/Phosphorylcholine. Expressed in Mammalian cells.
Species reactivity
General
Applications
ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Host species
Human
Clonality
Monoclonal
Isotype
IgG
Clone ID
ATH3G10
Biological activity
Phosphorylcholine is a component of drug delivery micelles, which improves the efficiency of cellular internalization and increases the cytotoxicity of cytotoxins carried in micelles in HeLa cells [2]. Phosphorylcholine (200-600 umol/kg; ip; single dose) activates nicotinic and muscarinic cholinergic neurotransmission, stimulates the activation of α2-adrenergic receptors, and increases plasma glucagon and choline concentrations, thereby stimulating peripheral autonomic nervous system activity [3]. Phosphorylcholine (2 ug; ip; once every two weeks for 4 months) induces the production of anti-PC and anti-oxLDL antibodies, increases mature B cells in the spleen, and reduces atherosclerotic lesions in the aortic root in mouse models [4].
Expression system
Mammalian cells
Nature
Recombinant
Target
Phosphocholine, Phosphorylcholine, ChoP
Endotoxin level
< 10 EU/mg as determined by LAL method.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
ChEBI: 18132, CAS: 107-73-3
Form
Liquid
Storage buffer
0.01M PBS pH 7.4.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
ATH3G10, ATH3G10, ATH-3G10
Background
ChoP/Phosphorylcholine is a protein, also known as ATH3G10, ATH3G10, ATH-3G10. Phosphorylcholine is a component of drug delivery micelles, which improves the efficiency of cellular internalization and increases the cytotoxicity of cytotoxins carried in micelles in HeLa cells [2]. Phosphorylcholine (200-600 umol/kg; ip; single dose) activates nicotinic and muscarinic cholinergic neurotransmission, stimulates the activation of α2-adrenergic receptors, and increases plasma glucagon and choline concentrations, thereby stimulating peripheral autonomic nervous system activity [3]. Phosphorylcholine (2 ug; ip; once every two weeks for 4 months) induces the production of anti-PC and anti-oxLDL antibodies, increases mature B cells in the spleen, and reduces atherosclerotic lesions in the aortic root in mouse models [4].
References
1. Harnett W, et al. Phosphorylcholine: friend or foe of the immune system? Immunol Today. 1999 Mar;20(3):125-9. [HP719016] 2. Tu S, et al., Enhancement of cellular uptake and antitumor efficiencies of micelles with phosphorylcholine. Macromol Biosci. 2011 Oct 10;11(10):1416-25. [HP719016] 3. Cansev M, et al., Choline, CDP-choline or phosphocholine increases plasma glucagon in rats: involvement of the peripheral autonomic nervous system. Eur J Pharmacol. 2008 Jul 28;589(1-3):315-22. [HP719016] 4. Caligiuri G, et al., Phosphorylcholine-targeting immunization reduces atherosclerosis. J Am Coll Cardiol. 2007 Aug 7;50(6):540-6. View More [HP719016]
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
SDS-PAGE
SDS-PAGE for Research Grade Anti-ChoPPhosphorylcholine Antibody (ATH3G10)
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