| Catalog No. | HP435016 |
|---|
| Species reactivity | Human |
|---|
| Applications | ELISA, Bioactivity: FACS, Functional assay, Research in vivo |
|---|
| Host species | Human |
|---|
| Isotype | IgG1-kappa |
|---|
| Clone ID | ALD1613 |
|---|
| Biological activity |
Adrenocorticotropic hormone (100 nM, 5weeks) induces clusters development of spermatogenesis isolated from mouse seminiferous tubules and significantly increases the proportion of pre-meiotic cells (CDH-1, VASA, MC2R-positive) and the expression of PLZF, VASA, MC2R, THY-1 genes [2]. Adrenocorticotropic hormone (100 nM, 5weeks) significantly increases the proportion and gene expression of meiotic marker BOULE-positive cells in cells isolated from mouse seminiferous tubules and has no significant effect on the proportion of meiotic marker CREM-positive cells, increases the proportion of post-meiotic marker ACROSIN-positive cells but decreases its gene expression, and has no effect on the expression of SCP3 and PROTAMINE [2]. Adrenocorticotropic hormone (1.25×10 -4 -2.5×10 -4 U/ml, 48 h) inhibits phagocytic function in MSU-stimulated THP-1-induced macrophages, promotes the polarization of MSU-stimulated macrophages to M2 type, downregulates the secretion of M1-type proinflammatory factors IL-1β, IL-6, and TNF-α, inhibit ROS production and reduces the opening of mitochondrial permeability transition pores (mPTP) to protect mitochondrial function [3]. Adrenocorticotropic hormone (1.25×10 -4 U/ml, 48 h) regulates the transcription of inflammation-related genes by upregulating genes such as NR1H2, IL-37, and FccRIIIA, and downregulating genes such as NLRP3, MC2R, and MC3R in THP-1-induced macrophages [3]. Adrenocorticotropic hormone (0.25-2.5 U/mL, s.c., 30 minutes after MSU intra-articular injection) significantly alleviates joint swelling, reduces inflammatory cell infiltration in joint tissues, and has no significant effect on serum cortisol levels in gout mice models [3]. |
| Expression system |
Mammalian cells |
| Clonality |
Monoclonal |
| Target |
Melanotropin alpha, Beta-MSH, Gamma-MSH, Beta-LPH, Melanotropin beta, Adrenocorticotropic hormone, Pro-opiomelanocortin, ACTH, CLIP, Gamma-LPH, Corticotropin-lipotropin, Alpha-MSH, POMC |
| Endotoxin level |
Please contact the lab for this information. |
| Purity |
>95% purity as determined by SDS-PAGE. |
| Purification |
Protein A/G purified from cell culture supernatant. |
| Accession |
P01189 |
| Form |
Liquid |
| Storage buffer |
0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
|
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | ALD1613 |
|---|
| Background | Pro-opiomelanocortin (ACTH) is a ~29 kDa protein. Precursor protein of pituitary hormones that are involved in diverse physiological processes, including the regulation of energy balance, stress response, immune function and skin pigmentation. |
|---|
| References |
1. Yamamoto R, et al.. Physiology, Adrenocorticotropic Hormone (ACTH). 2025 Dec 1. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025 Jan–. [HP435016]
2. AbuMadighem A, et al. Adrenocorticotropic hormone and its receptor as a novel testicular system involves in the development of spermatogenesis. Life Sci. 2025 May 1;368:123480. [HP435016]
3. Xu R, et al. Natural Adrenocorticotropic Hormone (ACTH) Relieves Acute Inflammation in Gout Patients by Changing the Function of Macrophages. J Healthc Eng. 2022 Mar 22;2022:9241835. [HP435016]
4. Bomback AS, et al. Treatment of nephrotic syndrome with adrenocorticotropic hormone (ACTH). Discov Med. 2011 Aug;12(63):91-6. View More [HP435016] |
| Note |
For research use only. Not suitable for clinical or therapeutic use. |
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