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Anti-Human ALPPL2 Reference Antibody (SGN-ALPV, RUO) (HY527016)

Anti-Human ALPPL2 Reference Antibody (SGN-ALPV, RUO)
Anti-Human ALPPL2 Reference Antibody (SGN-ALPV, RUO)
Anti-Human ALPPL2 Reference Antibody (SGN-ALPV, RUO)
Anti-Human ALPPL2 Reference Antibody (SGN-ALPV, RUO)
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Overview
Catalog No.HY527016
Description
Anti-Human ALPP/ALPPL2 Antibody (SGN-ALPV) (HY527016) is a research-grade recombinant antibody targeting PLAP-1. Produced in mammalian cells with native-like glycosylation.
Highlights
  • Research Grade — For PK/PD studies, assay development, and ADA research.
  • Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivityHuman
ApplicationsELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host speciesHuman
IsotypeIgG1
Clone IDSGN-ALPV
Expression system Mammalian cells
Clonality Monoclonal
Target PLAP-1, ALPP, Placental alkaline phosphatase 1, PLAP, Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type, Placental alkaline phosphatase-like, PLAP-like, ALPPL2, Alkaline phosphatase, placental-like, ALP-1, GCAP, Germ cell alkaline phosphatase, ALPPL, Alkaline phosphatase Nagao isozyme, ALPG, Alkaline phosphatase, germ cell type
Endotoxin level Please contact the lab for this information.
Purity >95% purity as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P05187, P10696
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Alkaline phosphatase, placental type (ALPP/PLAP) is a ~57 kDa protein. Alkaline phosphatase that can hydrolyze various phosphate compounds.

Note For research use only. Not suitable for clinical or therapeutic use.
Images
  • Anti-Human ALPPL2 Reference Antibody (SGN-ALPV, RUO)

    SDS-PAGE

    SDS-PAGE for Research Grade Anti-Human ALPP/ALPPL2 Antibody (SGN-ALPV)

  • Anti-Human ALPPL2 Reference Antibody (SGN-ALPV, RUO)

    Flow cytometry

    Flow-cytometry using anti-human ALPP & ALPG antibody. HPAC cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human ALPP & ALPG monoclonal antibody (Catalog HY527016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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