Anti-Human CD127/IL7R Reference Antibody (GSK2618960, RUO) (HB016046)

Name: Anti-Human CD127/IL7R Reference Antibody (GSK2618960, RUO)
Brand: abinScience
SKU: HB016046
Price: 176 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HB016046
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Clone ID	GSK2618960
Biological activity	GSK2618960 (10-100 ug/mL; 10 h) potently induces dose-dependent CD4 + T cell proliferation in PBMCs [2]. GSK2618960 binds specifically to monocyte-derived DCs, but not to uncultured primary DCs, NK cells, or B cells from fresh PBMCs [2]. GSK2618960 (50 ug/mL; 7 days) enhances the differentiation of DCs and upregulates their activation marker (CD83, CD86 and CD209) expression in PBMCs [2].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	CD127, CDw127, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7R-alpha, IL-7RA, IL7R, Interleukin-7 receptor subunit alpha
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P16871
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	GSK2618960
Background	Interleukin-7 receptor subunit alpha (CD127/IL7R) is a ~51 kDa protein. Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
References	1. Ellis J, et al. Anti-IL-7 receptor α monoclonal antibody (GSK2618960) in healthy subjects - a randomized, double-blind, placebo-controlled study. Br J Clin Pharmacol. 2019;85(2):304-315. [HB016046] 2. Liao K, et al. Characterization of the robust humoral immune response to GSK2618960, a humanized anti-IL-7 receptor monoclonal antibody, observed in healthy subjects in a Phase 1 study. PLoS One. 2021;16(3):e0249049. Published 2021 Mar 23. [HB016046]
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

Detects Human CD127/IL7R in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD127/IL7R (GSK2618960)
Flow cytometry

Flow-cytometry using APC anti-human CD127 antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD127 monoclonal antibody (Catalog HB016046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human CD127 antibody. 293T transfected cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD127 monoclonal antibody (Catalog HB016046, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.