Anti-Human CD156b/ADAM17 Reference Antibody (MEDI3622, RUO) (HX200016)

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100ug
1mg

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Datasheet

Overview

Catalog No.	HX200016
Description	Anti-Human CD156b/ADAM17 (MEDI3622) (HX200016) is a research-grade recombinant antibody targeting CD156. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Clone ID	MEDI3622
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	TACE, Snake venom-like protease, ADAM17, CD156b, CSVP, ADAM 17, Disintegrin and metalloproteinase domain-containing protein 17, TNF-alpha convertase, TNF-alpha-converting enzyme
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P78536
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names	MEDI3622
Background	Disintegrin and metalloproteinase domain-containing protein 17 (CD156b/ADAM17) is a ~93 kDa protein. Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins including adhesion proteins, growth factor precursors and cytokines important for inflammation and immunity. Cleaves the membrane-bound precursor of TNF to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). 1. Boskovski, MT. et al. (2013) Nature 504, 456-9. PMID: 24226769 2. Heurich, A. et al. (2014) Journal of virology 88, 1293-307. PMID: 24227843 3. Riethmueller, S. et al. (2017) PLoS biology 15, e2000080. PMID: 28060820 4. Feuerbach, D. et al. (2017) Neuroscience letters 660, 109-114. PMID: 28923481 5. Müller, M. et al. (2022) Cells 11. PMID: 36078095 6. Moss, ML. et al. (1997) Nature 385, 733-6. PMID: 9034191 7. Rabquer, BJ. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 1777-85. PMID: 20592283 8. Thathiah, A. et al. (2003) The Journal of biological chemistry 278, 3386-94. PMID: 12441351
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD156b/ADAM17 (MEDI3622).
Bioactivity

Detects Human CD156b/ADAM17 in indirect ELISAs.
Western blot

Western blot analysis was performed using anti-CD156b/ADAM17 monoclonal antibody at 1µg/ml on various samples.
Lane 1: recombinant human CD156b/ADAM17 (Catalog No: HX200012)
Lane 2: negative control