Research Grade Anti-Human CD203c/ENPP3 (AGS-16C3F) (HT018016)

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100ug
1mg

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Datasheet

Overview

Catalog No.	HT018016
Description	Anti-Human CD203c/ENPP3 (AGS-16C3F) (HT018016) is a research-grade recombinant antibody targeting CD203. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG
Clone ID	AGS-16C3F
Expression system	Mammalian Cells
Clonality	Monoclonal
Target	NPPase, PD-Ibeta, Phosphodiesterase I beta, PDNP3, CD203c, Ectonucleotide pyrophosphatase/phosphodiesterase family member 3, E-NPP 3, ENPP3, NPP3, Phosphodiesterase I/nucleotide pyrophosphatase 3, Nucleotide diphosphatase
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	O14638
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternate Names	AGS-16C3F, AGS16C3F, AGS16C3F
Background	Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (CD203c/ENPP3) is a ~100 kDa protein. Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Also hydrolyzes extracellular 2',3'-cGAMP (cyclic GMP-AMP), a second messenger that activates TMEM173/STING and triggers type-I interferon production, and is therefore involved in the regulation of innate immune response. 2',3'-cGAMP appears to be further processed to GMP, AMP and inorganic phosphate. Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. 1. Gorelik, A. et al. (2018) The FEBS journal 285, 2481-2494. PMID: 29717535 2. Jin-Hua, P. et al. (1997) Genomics 45, 412-5. PMID: 9344668 3. Mardjuki, R. et al. (2024) Cell reports 43, 114209. PMID: 38749434 4. Vollmayer, P. et al. (2003) European journal of biochemistry 270, 2971-8. PMID: 12846830 5. Bühring, HJ. et al. (2001) Blood 97, 3303-5. PMID: 11342463
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Flow cytometry

HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-CD203c monoclonal antibody (Catalog HT018016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analyzed on a NovoCyte Flow Cytometer.
Flow cytometry

Untransfected cells or cells transfected with CD203c/ENPP3 were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD203c monoclonal antibody (Catalog HT018016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analyzed on a NovoCyte Flow Cytometer.