Anti-Human EpCAM/TROP1 Reference Antibody (ING-1, RUO) (HB104136) is a human monoclonal antibody detecting EpCAM/TROP1.
Species reactivity
Human
Applications
ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Host species
Human
Clonality
Monoclonal
Isotype
IgG1
Clone ID
ING-1
Biological activity
activity, targeting and inhibiting tumor growth and metastasis in mouse cancer models. ING-1 is useful in the research of breast, colorectal, and lung cancers, among other cancers [1] [2]. [1]. ING-1(37°C for 4 h) caused a concentration-dependent lysis of 51 Cr-labeled BT-20, MCF-7, HT-29, CACO-2, NCI-H1568, PC-3, HPAF-II cells in ADCC and CDC activity assay [1]. Parmacokinetics Species Dose Route V ss CL MRT T 1/2 T max C max Rat [1] 0.5 mg/kg i.v. 108 4.43 24.5 12.9 / / Rat [1] 5 mg/kg s.c. / 7.95 25.9 29.4 4.94 21.5 Rat [1] 50 mg/kg i.v. 114 4.56 25 5.85 / / ING-1 (0.1-1.0 mg/kg, i.v., two doses per week, 3 weeks) reduces tumor size and metastases in HT-29 and PC-3 xenograft nude mice model [1][2].
Protein A/G purified from cell culture supernatant.
Accession
P16422
Form
Liquid
Storage buffer
0.01M PBS pH 7.4.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
ING-1, ING1, ING1
Background
Epithelial cell adhesion molecule (CD326/EPCAM) is a ~34 kDa protein. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
References
1. Ammons WS, et al. In vitro and in vivo pharmacology and pharmacokinetics of a human engineered monoclonal antibody to epithelial cell adhesion molecule. Neoplasia. 2003 Mar-Apr;5(2):146-54. [HB104136] 2. Ruan HH, et al. ING-1(heMAb), a monoclonal antibody to epithelial cell adhesion molecule, inhibits tumor metastases in a murine cancer model. Neoplasia. 2003 Nov-Dec;5(6):489-94. [HB104136]
Note
For research use only. Not suitable for clinical or therapeutic use.
SDS-PAGE for Research Grade Anti-Human CD326/EPCAM (ING-1).
Flow cytometry
Flow-cytometry using anti-human CD326 antibody. HT29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD326 monoclonal antibody (Catalog HB104136, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
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