Anti-Human FGFR1 Reference Antibody (OM-RCA-01, RUO) (HY105026)

Name: Anti-Human FGFR1 Reference Antibody (OM-RCA-01, RUO)
Brand: abinScience
SKU: HY105026
Price: 176 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HY105026
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Clone ID	OM-RCA-01
Biological activity	OM-RCA-01 binds to FGFR1 with high affinity (K d = 1.59 nM) and inhibits its in vitro kinase activity [1]. OM-RCA-01 (1-100 ug/mL, 3 h) potently inhibits FGFR1 phosphorylation and cell proliferation in FGFR1-overexpressing human lung cancer cell line A549 (GI 50 = 9.69 ug/mL), but exerts no significant effect on FGFR1-low-expressing human melanoma cell line Mel Kor [1]. OM-RCA-01 (0.5-10 nmol/L; 5 days) enhances Nivolumab -induced IFN-γ release and T-cell proliferation in a human allogeneic MLR assay, whereas it exhibits minimal effects when used alone when combined with Nivolumab () [1]. OM-RCA-01 (1 ug/mL; 4 days) increases Nivolumab -induced IL-2 secretion by 29%-74% in human peripheral blood mononuclear cells (PBMCs) stimulated with Staphylococcal enterotoxin B (SEB) () [1]. Western Blot Analysis [1] Cell Line: A549 cells Concentration: 1-100ug/mL Incubation Time: 3 h Result: Resulted in a dose-dependent suppression of FGFR1 phosphorylation. Parmacokinetics Species Dose Route C 0 T 1/2 V d CL AUC 0-t AUC 0-∞ MRT Rat [1] 10 mg/kg i.v. 186 ng/mL 121 h 532 L 3.05 L/h 10665 ng·h/mL 32818 ng·h/mL 34 h Rat [1] 30 mg/kg i.v. 177 ng/mL 136 h 2533 L 12.88 L/h 14237 ng·h/mL 46602 ng·h/mL 34 h Rat [1] 100 mg/kg i.v. 245 ng/mL 234 h 7569 L 22.44 L/h 16383 ng·h/mL 89131 ng·h/mL 35 h OM-RCA-01 (30 mg/kg; i.v.; twice weekly) induces a statistically significant two-fold reduction in median tumor volume and delays tumor growth in A549 lung cancer xenograft models, and combination with Pembrolizumab () achieves 89% tumor growth inhibition [1]. OM-RCA-01 (30 mg/kg; once every 3 days) achieves 96% tumor growth delay in the cancer-associated fibroblast (CAF)-positive renal cell carcinoma cohort and 75% tumor growth delay in the CAF-negative renal cell carcinoma cohort, while increasing IFN-γ and IL-2 cytokine levels [1].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	FLT2, HBGFR, BFGFR, FGFR-1, CEK, Fibroblast growth factor receptor 1, FGFR1, bFGF-R-1, Fms-like tyrosine kinase 2, FGFBR, FLT-2, CD331, Basic fibroblast growth factor receptor 1, FLG, Proto-oncogene c-Fgr, N-sam
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P11362
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	OM-RCA-01
Background	Fibroblast growth factor receptor 1 (CD331/FGFR1) is a ~91 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate.
References	1. Tsimafeyeu I, et al. A novel anti-FGFR1 monoclonal antibody OM-RCA-01 exhibits potent antitumor activity and enhances the efficacy of immune checkpoint inhibitors in lung cancer models. Immunooncol Technol. 2024;23:100725. Published 2024 Jul 26. [HY105026]
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD331/FGFR1 (OM-RCA-01).
Flow CytoMetry

Flow-cytometry using PE anti-human CD331 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD331 monoclonal antibody (Catalog: HY105026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human FGFR1 Reference Antibody (OM-RCA-01, RUO).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human FGFR1 Reference Antibody (OM-RCA-01, RUO) (HY105026)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us