Anti-Human CD3 epsilon Reference Antibody (IOR-T3, RUO) (HY057096)

Name: Anti-Human CD3 epsilon Reference Antibody (IOR-T3, RUO)
Brand: abinScience
SKU: HY057096
Price: 370 USD
Availability: InStock

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Overview

Catalog No.	HY057096
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG
Clone ID	IOR-T3
Biological activity	IOR-T3 (1 h) potently binds to CD3 on human PBMCs, with equivalent antigen-binding capacity to its chimeric variants T3q and T3q(Ala=Ala) [1]. IOR-T3 (70-100 ug/mL; 7 days) does not inhibit the growth of human non-small-cell lung carcinoma H125 cells when used at 100 ug/mL for 7 days [2]. Cell Proliferation Assay [2] Cell Line: human non-small-cell lung carcinoma H125 cells Concentration: 70, 90, 100 ug/mL Incubation Time: 7 days Result: Showed no inhibitory effect on H125 cell growth, with cell protein content remaining at 100% of control levels.
Expression system	Mammalian cells
Clonality	Monoclonal
Target	T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain
Endotoxin level	< 10 EU/mg
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P07766
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	IOR-T3, IORT3, IORT3
Background	T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto). 1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938 2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002 3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098 4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325 5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660
References	1. Hinojosa LE, et al. Construction of a recombinant non-mitogenic anti-human CD3 antibody. Hybridoma (Larchmt). 2010;29(2):115-124. [HY057096] 2. Suarez Pestana E, et al. Growth inhibition of human lung adenocarcinoma cells by antibodies against epidermal growth factor receptor and by ganglioside GM3: involvement of receptor-directed protein tyrosine phosphatase(s). Br J Cancer. 1997;75(2):213-220. [HY057096]
Note	For research use only. Not suitable for clinical or therapeutic use.