Anti-Human P-Cadherin Reference Antibody (PCA062, RUO) (HB245036)

Name: Anti-Human P-Cadherin Reference Antibody (PCA062, RUO)
Brand: abinScience
SKU: HB245036
Price: 176 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HB245036
Description	Anti-Human CDH3/P-cadherin (PCA062) (HB245036) is a research-grade recombinant antibody targeting Placental cadherin. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1
Clone ID	PCA062
Expression system	Mammalian cells
Clonality	Monoclonal
Tested applications	FCM
Target	Placental cadherin, CDH3, Cadherin-3, P-cadherin, CDHP
Endotoxin level	< 10 EU/mg
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P22223
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	PCA062
Background	Cadherin-3 (CDH3/P-cadherin) is a ~91 kDa protein. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CDH3/P-cadherin (PCA062).
Flow CytoMetry

Flow-cytometry using FITC anti-human CDH3 antibody. A431 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CDH3 monoclonal antibody (Catalog: HB245036, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CDH3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CDH3 monoclonal antibody (Catalog: HB245036) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.