Anti-Human CXCL12/SDF-1 beta Reference Antibody (Genentech anti-CXCL12, RUO) (HW376016)

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100ug
1mg

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Catalog No.	HW376016
Description	Anti-Human CXCL12/SDF-1 Antibody (Genentech anti-CXCL12) (HW376016) is a research-grade recombinant antibody targeting Intercrine reduced in hepatomas. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG
Clone ID	Genentech anti-CXCL12
Expression system	Mammalian Cells
Target	Intercrine reduced in hepatomas, hSDF-1, Stromal cell-derived factor 1, SDF-1, Pre-B cell growth-stimulating factor, SDF1, SDF1A, C-X-C motif chemokine 12, SDF1B, CXCL12, IRH, PBSF, hIRH
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P48061
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Stromal cell-derived factor 1 (CXCL12/SDF-1) is a ~10 kDa protein. Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for CXCL12/SDF-1. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. 1. Malik, M. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 181, 4632-7. PMID: 18802065 2. Liu, Y. et al. (2024) Cell reports 43, 114578. PMID: 39093700 3. Oberlin, E. et al. (1996) Nature 382, 833-5. PMID: 8752281 4. Balabanian, K. et al. (2005) The Journal of biological chemistry 280, 35760-6. PMID: 16107333 5. Kalatskaya, I. et al. (2009) Molecular pharmacology 75, 1240-7. PMID: 19255243 6. Fujita, M. et al. (2018) The Biochemical journal 475, 723-732. PMID: 29301984
Note	For research use only. Not suitable for clinical or therapeutic use.

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Anti-Human CXCL12/SDF-1 beta Reference Antibody (Genentech anti-CXCL12, RUO).pdf

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Anti-Human CXCL12/SDF-1 beta Reference Antibody (Genentech anti-CXCL12, RUO) (HW376016)

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