Anti-Human IL-1 beta/IL-1F2 Reference Antibody (SK48-E26, RUO) (HF943086)

Name: Anti-Human IL-1 beta/IL-1F2 Reference Antibody (SK48-E26, RUO)
Brand: abinScience
SKU: HF943086
Price: 370 USD
Availability: InStock

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Overview

Catalog No.	HF943086
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG
Clone ID	SK48-E26
Biological activity	SK48-E26 effectively captures human recombinant IL-1β in an ELISA format, enabling detection of hrIL-1β at concentrations as low as 0.1 to 0.2 ng/mL with a linear response to IL-1β concentration [1]. SK48-E26 specifically neutralizes human recombinant IL-1β activity in an EL-4 cell-based bioassay, binds a conformational epitope on hrIL-1β spanning amino acids 95-101, and has an affinity for IL-1β of 0.5 to 3 nM [1]. Mutations in the heavy chain CDR3 region of SK48-E26 Fab fragments increase affinity for human recombinant IL-1β, with the V120T/R121K mutant showing a 9.79-fold improvement over the wild-type SK48-E26 Fab [1]. SK48-E26 (10-200 nM) binds to human IL-1β with an equilibrium dissociation constant of 242 pM [2]. SK48-E26 (1×10 -15 -1×10 -7 M; 1 h pre-incubation with IL-1β, 16-20 h incubation with cells) neutralizes human IL-1β with an IC 50 of 400 pM and cynomolgus IL-1β with an IC 50 of 185 pM in MRC-5 cells [2].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	Interleukin-1 beta, IL1B, Catabolin, IL1F2, IL-1 beta
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P01584
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	SK48-E26, SK48E26, SK48E26
Background	Interleukin-1 beta (IL1B/IL1F2) is a ~30 kDa protein. Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. IL1B is the therapeutic target of canakinumab (Ilaris). 1. Tominaga, K. et al. (2000) International immunology 12, 151-60. PMID: 10653850 2. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819 3. LaRock, CN. et al. (2016) Science immunology 1. PMID: 28331908 4. Van Damme, J. et al. (1985) Nature 314, 266-8. PMID: 3920526 5. Tian, XX. et al. (2021) International endodontic journal 54, 916-925. PMID: 33377178 6. Xia, S. et al. (2021) Nature 593, 607-611. PMID: 33883744
References	1. Peter Ronald Young, et al. RECOMBINANT AND HUMANIZED IL-1β ANTIBODIES FOR TREATMENT OF IL-1 MEDIATED INFLAMMATORY DISORDERS IN MAN. WO1995001997A1. 1994. [HF943086] 2. Lacy SE, et al. Generation and characterization of ABT-981, a dual variable domain immunoglobulin (DVD-Ig(TM)) molecule that specifically and potently neutralizes both IL-1α and IL-1β. MAbs. 2015;7(3):605-19. [HF943086]
Note	For research use only. Not suitable for clinical or therapeutic use.