Anti-Human BCMA/TNFRSF17&CD3 epsilon Reference Antibody (Vonsetamig, RUO) (HX959196)

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Price(USD): $

Spec:

100ug
1mg

Number:

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX959196

Description

Vonsetamig (HX959196) is a research-grade recombinant antibody targeting CD269. Produced in mammalian cells with native-like glycosylation.

Highlights

●Research Grade — For PK/PD studies, assay development, and ADA research.
●Native Glycosylation — Mammalian expression ensures native-like patterns.

Species reactivity

Human

Applications

ELISA, Bioactivity: FACS, Functional assay, Research in vivo, WB

Host species

Human

Isotype

IgG4-kappa

Expression system

Mammalian Cells

Clonality

Monoclonal

Tested applications

Target

Tumor necrosis factor receptor superfamily member 17, TNFRSF17, CD269, B-cell maturation protein, BCMA, BCM, T3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T-cell surface glycoprotein CD3 epsilon chain

Endotoxin level

Please contact the lab for this information.

Purity

>95% purity as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q02223 & P07766

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

2408319-33-3

Background

Teclistamab is an active T cell–redirecting bispecific antibody against B-cell maturation antigen for multiple myeloma

Note

For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Vonsetamig.
Flow cytometry

Flow-cytometry using anti-human CD3E antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD3E monoclonal antibody (Catalog HX959196, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using anti-human CD269 antibody. Transfected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD269 monoclonal antibody (Catalog HX959196, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects recombinant CD269 (Catalog No: HX959012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-CD3E monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD3E (Catalog No: HY057021)
Lane 2: Negative control