The Strep-tag system — based on the octapeptide WSHPQFEK (Strep-tag II) and its tandem variant Twin-Strep-tag — is one of the most widely used affinity purification tags in recombinant protein research. Strep-tag binds Strep-Tactin with high specificity under physiological conditions, enabling gentle, one-step purification without denaturing elution. Reliable anti-Strep Tag antibodies are essential for confirming tag presence by western blot, validating expression in cell lysates, and detecting tagged proteins in immunofluorescence or flow cytometry.
abinScience provides recombinant anti-Strep Tag antibodies in multiple formats — unconjugated, HRP-conjugated, and Twin-Strep-tag-specific — along with control proteins for assay standardization.
Why abinScience?
✓ Recombinant consistency — Lot-to-lot reproducibility for tag detection across experiments
✓ Multiple clones — C23.21, 3E8#, 1A188, 1A189 — test for optimal signal in your system
✓ HRP conjugate available — Direct detection in western blot without secondary antibody
✓ Twin-Strep-tag clone — SAA0348 specifically recognizes the tandem Twin-Strep format
Available Products
Anti-Strep Tag Recombinant & Monoclonal Antibodies
Recombinant and monoclonal antibodies recognizing the Strep-tag II peptide (WSHPQFEK). Available unconjugated and HRP-conjugated.
Strep Tag Control Proteins
Recombinant control proteins for assay validation and positive controls.
About the Target
Tag sequence: WSHPQFEK (Strep-tag II) / WSHPQFEK-GGGSGGGSGGS-WSHPQFEK (Twin-Strep-tag)
Binding partner: Strep-Tactin (engineered streptavidin)
Elution: Biotin or desthiobiotin (physiological pH, no denaturing)
The Strep-tag II system was developed as a gentle alternative to His-tag/IMAC purification. Its key advantages include minimal non-specific binding to endogenous proteins, compatibility with cell-surface display, and suitability for purifying protein complexes under native conditions. The Twin-Strep-tag variant provides enhanced binding affinity (Kd in the low nanomolar range) for applications requiring higher stringency.
Frequently Asked Questions
What is Strep-tag II?
Strep-tag II is an eight-amino-acid peptide (WSHPQFEK) engineered for high-affinity binding to Strep-Tactin, a modified streptavidin. It is fused to recombinant proteins to enable one-step affinity purification under native, non-denaturing conditions.
What is the difference between Strep-tag and Twin-Strep-tag?
Twin-Strep-tag consists of two Strep-tag II sequences connected by a short linker, providing substantially higher binding affinity to Strep-Tactin XT. This increases purification yield and allows more stringent washing without losing target protein. Use the Twin-Strep-tag-specific antibody (SAA0348) for detection.
Which clone should I use for western blot?
For direct WB detection without secondary antibody, use the HRP-conjugated clone 1A188 (YP396315). For indirect WB with a secondary antibody, any of the unconjugated clones will work — C23.21 and 1A188 are good starting points.
Need Help Selecting the Right Product?
Our scientists can help match the right antibody or protein to your application.
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All products are for research use only (RUO). Not intended for diagnostic or therapeutic use. Product availability and specifications are subject to change. Visit individual product pages for the most current information.
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