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InVivoMAb Anti-Human TNF-alpha Antibody (Iv0050) (HF879010)

InVivoMAb Anti-Human TNF-alpha Antibody (Iv0050)
InVivoMAb Anti-Human TNF-alpha Antibody (Iv0050)
InVivoMAb Anti-Human TNF-alpha Antibody (Iv0050)
InVivoMAb Anti-Human TNF-alpha Antibody (Iv0050)
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Spec:
  • 100ug
  • 1mg
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  • Overview

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  • References

  • Datasheet

  • SDS

Overview
Catalog No.HF879010
Description
InVivoMAb Anti-Human TNFa/TNF-alpha (Iv0050) [Iv0050] (HF879010) is a human monoclonal antibody detecting TNF in ELISA, FCM, Neutralization. Suitable for Human.
Highlights
  • ●Multi-Application — Validated across multiple applications.
  • ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivityHuman
ApplicationsELISA, FCM, Neutralization
Host speciesHuman
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone IDIv0050
Tested applications FCM
Target C-domain 1, C-domain 2, Cachectin, ICD1, ICD2, Intracellular domain 1, Intracellular domain 2, N-terminal fragment, NTF, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tumor necrosis factor, membrane form, Tumor necrosis factor, soluble form
Endotoxin level < 1 EU/mg
Purity >95% as determined by SDS-PAGE.
Associated products pH 7.4 Dilution Buffer
Purification Protein A/G purified from cell culture supernatant.
Accession P01375
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note For research use only. Not suitable for clinical or therapeutic use.
Isotype Control Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)
Images
  • InVivoMAb Anti-Human TNF-alpha Antibody (Iv0050)

    Flow Cytometry

    Flow-cytometry using anti-human TNFa antibody.TNFa Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa antibody monoclonal antibody (Catalog # HF879010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • InVivoMAb Anti-Human TNF-alpha Antibody (Iv0050)

    SDS-PAGE

    SDS-PAGE for InVivoMAb Anti-Human TNFa/TNF-alpha (Iv0050).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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