Anti-Human CD81 Antibody (005-C01) (HX855013)

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Datasheet

Overview

Catalog No.

HX855013

Description

Anti-Human CD81 Antibody (005-C01) [005-C01] (HX855013) is a human monoclonal antibody detecting CD81 in ELISA, FCM, IHC, WB. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

ELISA, FCM, IHC, WB

Host species

Human

Isotype

IgG1

Clone ID

005-C01

Clonality

Monoclonal

Target

CD81 antigen, TSPAN28, Target of the antiproliferative antibody 1, Tspan-28, Tetraspanin-28, CD81, TAPA1, 26 kDa cell surface protein TAPA-1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P60033

RRID

Anti-Human CD81 Antibody (005-C01) (abinScience Cat# HX855013, RRID:AB_3729721)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

CD81 antigen is a ~25 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Essential for trafficking and compartmentalization of CD19 receptor on the surface of activated B cells. Upon initial encounter with microbial pathogens, enables the assembly of CD19-CR2/CD21 and B cell receptor (BCR) complexes at signaling TERMs, lowering the threshold dose of antigen required to trigger B cell clonal expansion and antibody production. In T cells, facilitates the localization of CD247/CD3 zeta at antigen-induced synapses with B cells, providing for costimulation and polarization toward T helper type 2 phenotype. Present in MHC class II compartments, may also play a role in antigen presentation.

1. Shoham, T. et al. (2006) Molecular and cellular biology 26, 1373-85. PMID: 16449649

2. van Zelm, MC. et al. (2010) The Journal of clinical investigation 120, 1265-74. PMID: 20237408

3. Zimmerman, B. et al. (2016) Cell 167, 1041-1051.e11. PMID: 27881302

4. Cherukuri, A. et al. (2004) The Journal of biological chemistry 279, 31973-82. PMID: 15161911

5. Sagi, Y. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 1613-8. PMID: 22307619

6. Rocha-Perugini, V. et al. (2013) Molecular and cellular biology 33, 3644-58. PMID: 23858057

7. Secrist, H. et al. (1996) European journal of immunology 26, 1435-42. PMID: 8766544

8. Schick, MR. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 4090-7. PMID: 8409388

9. Takeda, Y. et al. (2003) The Journal of cell biology 161, 945-56. PMID: 12796480

Note

For research use only.

Images

SEC-HPLC

SEC-HPLC detection for Anti-Human CD81 Antibody (005-C01).
Bioactivity

Detects CD81 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD81 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD81 antibody monoclonal antibody (Catalog # HX855013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CD81 Antibody (005-C01)
Western blot

Western blot analysis was performed using anti-CD81 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD81 (Catalog No: HX855011)
Lane 2: negative control

References

Datasheet

Anti-Human CD81 Antibody (005-C01).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human CD81 Antibody (005-C01) (HX855013)

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