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Anti-Human GAPDH Monoclonal Antibody (1A200) (HY030035)

Anti-Human GAPDH Monoclonal Antibody (1A200)
Anti-Human GAPDH Monoclonal Antibody (1A200)
Anti-Human GAPDH Monoclonal Antibody (1A200)
Anti-Human GAPDH Monoclonal Antibody (1A200)
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Overview
Catalog No.HY030035
Description
Anti-Human GAPDH Monoclonal Antibody (1A200) [1A200] (HY030035) is a mouse monoclonal antibody detecting GAPDH in ELISA, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • Recombinant — Superior batch-to-batch reproducibility with defined sequences.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, WB
Host speciesMouse
IsotypeIgG2b
Clone ID1A200
Clonality Monoclonal
Immunogen E. coli - derived recombinant Human GAPDH protein (Met1-Glu335).
Target GAPD, Peptidyl-cysteine S-nitrosylase GAPDH, GAPDH, Glyceraldehyde-3-phosphate dehydrogenase
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Anti-Human GAPDH Monoclonal Antibody (1A200)

    SDS-PAGE

    SDS-PAGE for Anti-Human GAPDH Monoclonal Antibody (1A200)

  • Anti-Human GAPDH Monoclonal Antibody (1A200)

    Western blot

    Western blot analysis was performed using anti-GAPDH monoclonal antibody at 1ug/mL on various samples.
    Lane 1: MCF-7 cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: NIH3T3 cell lysate
    Lane 4: PC-12 cell lysate
    Lane 5: PC-3 cell lysate
    Lane 6: Mouse kidney lysate
    Lane 7: Rat spleen lysate
    Lane 8: Rat kidney lysate

Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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