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Home > Products > Antibodies > Flow Cytometry (FACS) Antibodies

Flow Cytometry (FACS) Antibodies

Build high-resolution multicolor flow cytometry panels with our panel-ready FACS antibodies. Choose from bright fluorophores (BV421, FITC, PE, APC, Alexa Fluor 488/647), fixation-compatible options, and validated clones with isotype, titration, and compensation guidance. Includes intracellular staining kits, Fc block, viability dyes, and FMO/isotype controls. RUO

Why Researchers Choose abinScience

Panel-Ready Pre-titrated, validated clones for 8+ color panels
Bright Fluors BV421/605, PE, APC, Alexa Fluor 488/647, tandems
Fixation-Stable Intracellular & surface markers post-PFA/formalin
Full Support Titration, compensation, FMO, spectral overlap data
Complete Kits Fc block, viability, isotype, compensation beads

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Selection Guide

  1. Antigen Density: Assign brightest dyes (PE, APC, BV421) to low-expression markers; dimmer dyes to high-expression.
  2. Spectral Overlap: Use spectral viewer; avoid high-spillover pairs (e.g., PE vs. PE-Cy7); include compensation controls.
  3. Fixation Needs: Surface markers → live or mild fix; intracellular → permeabilization kits (check fluor stability).
  4. Panel Design: Include FMO for each fluor, isotype controls, Fc block, viability dye; titrate each antibody.
  5. Instrument: Confirm laser/filter compatibility; use single-stained beads/cells for compensation matrix.

Applications

Immunophenotyping of PBMCs, spleen, bone marrow
Tumor-infiltrating lymphocyte (TIL) profiling
Intracellular cytokine staining (ICS) post-stimulation
Stem cell and progenitor analysis
Checkpoint inhibitor mechanism studies

Documents & QC

Clone, isotype, reactivity, recommended titration range
Representative FACS histograms and gating strategy
Compensation matrix, FMO examples, protocol PDFs
Lot-specific CoA; storage and light protection guidance

FAQs

How do I choose fluorophores for low-density antigens?

Assign the brightest dyes (PE, APC, BV421/BV605) to low-abundance markers; use medium/dimmer dyes for highly expressed markers.

FMO vs. isotype—when to use each?

FMOs define gating thresholds in multicolor panels; isotypes estimate non-specific binding but do not replace FMOs.

Do I need single-color compensation controls?

Yes, include single-color controls (cells or beads) for every fluor to generate accurate compensation matrices.

Are tandem dyes fixation-stable?

Most tandems tolerate common fixatives but can be sensitive to harsh conditions and storage; check product-specific compatibility.

RUO disclaimer

All antibodies are for Research Use Only (RUO) and not for diagnostic procedures.

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    CD132/IL2RG (5)
    TPO/Thyroidperoxidase (15)
    S1PR3 (5)
    STEAP2 (5)
    IFNGR2 (5)
    CDw210b/IL10RB (10)
    IgA2-lambda (1)
    GPR20 (5)
    BTN2A1 (5)
    BTN2A1/BTN2A2 (5)
    IgG4-kappa (2)
    CD18 & CD11 (5)
    Glycosaminoglycans (GAGs) (10)
    ADGRE1/F4/80 (5)
    IgG3-lambda (10)
    IgG4-lambda (10)
    IgG2-lambda (6)
    pstS1 (10)
    acp-1 (10)
    CD38/ADPRC1 (5)
    FLT4/VEGFR3 (5)
    IL11RA (2)
    CD36L2 (4)
    DPEP3 (5)
    CD77 (15)
    TF/CD176 (15)
    IgG1 CH1-IgG2 Fc Fragment (1)
    IgG1 (E356D/M358L) (1)
    IgG1 (Fab-His) (1)
    IgG1 (K214R) (1)
    IgG1 (K214R/K447A) (1)
    IgG1 (L234A/L235A) (2)
    IgG1 (L234F/L235E/P331S) (1)
    IgG1 (D265A) (2)
    IgG1 (D265A/N297A) (1)
    IgG1 (D270N) (1)
    IgG1 (E233A/L235A) (1)
    IgG1 (E356D/M358L/L234A/L235A) (1)
    IgG1 (E356D/M358L/L234A/L235A/F405L) (1)
    IgG1 (F405L) (1)
    IgG1 (K214R/E356D/M358L) (1)
    IgG1 (K214R/L234A/L235A) (1)
    IgG1 (K214R/L234F/L235E/P331S) (1)
    IgG1 (K214R/N297A) (1)
    IgG1 (K409R/E356D/M358L) (1)
    IgG1 (L234A/L235A/F405L) (1)
    IgG1 (L234A/L235A/G237A) (1)
    IgG1 (L234F/L235E) (1)
    IgG1 (N297A/E356D/M358L) (1)
    IgG1 (N297Q) (1)
    IgG1 (N297S) (1)
    IgG1 (P329G/E356D/M358L) (1)
    IgG1 (P331S/E430G) (1)
    IgG4 (S228P/R409K) (1)
    IgG4 (S228P/F234A/L235A) (2)
    IgG4 (S228P/D265A/L309V/R409K) (1)
    IgG4 (S228P/F234A/L235A/R409K) (1)
    IgG4 (S228P/L235E/D265A/R409K) (1)
    IgG4 (S228P/L235E/P329G) (1)
    IgG4 (S228P/L235E/R409K) (1)
    scFv-hFc (IgG1) (1)
    scFv-mFc (IgG2a) (1)
    scFv (1)
    VHH-mFc (IgG1) (1)
    VHH-mFc (IgG2a) (1)
    IgG2a (L234A/L235A) (1)
    IgG1 (M428L/N434S) (1)
    IgG1-G2-kappa (1)
    BiTE (Bispecific) (1)
    IgG (Bispecific) (3)
    IgD-kappa (1)
    FOXP3 (12)
    PDL1 (4)
    VMAT2 (3)
    CD327/SIGLEC6 (1)
    CD279/PDCD1/PD1 (1)
  • Species
    0 selected
    Mouse (8)
  • Applications
    0 selected
    FCM (5346)
    ELISA (1981)
    WB (543)
    Blocking (34)
    IP (47)
    IF (281)
    IHC (84)
    IHC-Fr (2)
    ICC (13)
    DB (5)
    SPR (21)
    RIA (7)
    Cell Isolation (1)
    Cell Depletion (2)
    FuncS (13)
    Neutralization (30)
    in vivo NK cell depletion (4)
    in vivo CD4+ T cell depletion (1)
    Isotype Control (356)
    In vivo Control (123)
    Block (2)
    T cell depletion (Reported) (1)
    T cell stimulation (Reported) (1)
    FCMin vivo CD40 activation (2)
    in vitro B cell stimulation/activation (2)
    IHC (P) (2)
    in vivo 4-1BB stimulation (2)
    in vitro 4-1BB stimulation (2)
    in vivo CTLA-4 neutralization (2)
    in vivo intra-tumoral regulatory T cell depletion (2)
    in vivo neutrophil depletion (2)
    in vivo MDSC depletion (2)
    in vivo IFNAR-1 blockade (2)
    in vitro IFNAR-1 blockade (2)
    Agonist (1)
    Fc Blocking (2)
    IHC(P) (1)
    IF (P) (8)
    IF-Fr (4)
    IF(P) (4)
    ICC-Fr (4)
    Control (4)
    in vivo CD8+ T cell depletion (1)
    Western blot (1)
  • Reactivity
    0 selected
    Mouse (734)
    Human (4348)
    Rat (87)
    Monkey (45)
    Dog (30)
    Bovine (21)
    Hamster (10)
    Chicken (5)
    Rabbit (5)
    Horse (6)
    Staphylococcus aureus (5)
    Pig (17)
    Human astrovirus-8 (HAtVs) (5)
    Baboon (5)
    General (25)
    Mycobacterium tuberculosis (20)
  • Host species
    0 selected
    mouse (3329)
    Human (1370)
    Rabbit (91)
    Rat (450)
    Hamster (10)
    Goat (5)
    Chicken (5)
    Llama (5)
    Armenian Hamster (15)
    Canine (8)
    Alpaca (10)
    Monkey (5)
    Dog (30)
    Feline (5)
    Guinea pig (5)
  • Clonality
    0 selected
    Monoclonal (5318)
    Polyclonal (45)
    Human (1)
  • Conjugate
    0 selected
    Unconjugated (1187)
    Alexa Fluor® 532 (1)
    FITC (1043)
    APC (1043)
    PerCP (1039)
    PE (1043)

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