Anti-GFP Recombinant Nanobody (SAA0958) (ZP144033)

Name: Anti-GFP Recombinant Nanobody (SAA0958)
Brand: abinScience
SKU: ZP144033
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

ZP144033

Description

Anti-GFP Nanobody (SAA0958) [SAA0958] (ZP144033) is a alpaca monoclonal antibody detecting GFP in ELISA. Suitable for Aequorea victoria.

Species reactivity

General

Applications

ELISA, IF

Host species

Alpaca

Clonality

Monoclonal

Isotype

VHH-8His-Cys-tag

Clone ID

SAA0958

Tested applications

Target

Green fluorescent protein, GFP

Endotoxin level

< 10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Green fluorescent protein (GFP) is a ~26 kDa protein. Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-GFP Nanobody (SAA0958)
Immunofluorescence

Immunofluorescent analysis of 4% PFA-fixed and 0.1% Triton X-100-permeabilized 293T cells transfected with pEGFP-N1 using VHH anti-GFP antibody (Cat. No. ZP144033, His-tagged) at 20 μg/mL. After incubation with mouse anti-His antibody, CY3-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

References

Datasheet

Anti-GFP Recombinant Nanobody (SAA0958).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-GFP Recombinant Nanobody (SAA0958) (ZP144033)

Overview

Images

References

Datasheet

SDS