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Anti-GFP Recombinant Nanobody (SAA0976) (ZP144053)

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Anti-GFP Recombinant Nanobody (SAA0976)
Anti-GFP Recombinant Nanobody (SAA0976)
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Overview
Catalog No.ZP144053
Description
Anti-GFP Nanobody (SAA0976) [SAA0976] (ZP144053) is a alpaca monoclonal antibody detecting GFP in ELISA. Suitable for Aequorea victoria.
Species reactivityGeneral
ApplicationsELISA
Host speciesAlpaca
ClonalityMonoclonal
IsotypeVHH-8His-Cys-tag
Clone IDSAA0976
Target Green fluorescent protein, GFP
Endotoxin level < 10 EU/mg
Purity >95% as determined by SDS-PAGE.
Purification Purified by Nickel column.
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Green fluorescent protein (GFP) is a ~26 kDa protein. Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Note For research use only.
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  • Anti-GFP Recombinant Nanobody (SAA0976)

    SDS-PAGE

    SDS-PAGE for Anti-GFP Nanobody (SAA0976)

References
Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-GFP Recombinant Nanobody (SAA0976)

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