The abinScience Tricolor Prestained Protein Marker is a ready-to-use mixture of nine (9) prestained proteins with apparent molecular weights ranging from 10 to 250 kDa. Three distinct colors — blue, orange, and green — enable rapid band identification on SDS-PAGE gels and easy monitoring of Western blot transfer efficiency. The marker is supplied in a ready-to-use format: simply load directly onto the gel with no heating, reduction, or addition of sample buffer required. Compatible with standard SDS-PAGE, Bis-Tris, Tris-Glycine, and Tris-Acetate gel systems. Detectable by colorimetric, NIR fluorescence (700 nm), and RGB fluorescence (555 nm) methods.
Applications
SDS-PAGE, Western Blot (WB)
Observed Molecular Weight
250, 130, 100, 70, 55, 35, 25, 15, 10 kDa
Form
Liquid
Storage buffer
62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃, 33% Glycerol
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Ready-to-use prestained protein molecular weight standard for SDS-PAGE and Western blot. Nine color-coded bands allow instant visual identification of molecular weight range during electrophoresis and real-time monitoring of protein transfer to membranes. Three reference bands (70 kDa — orange; 25 kDa — green; others — blue) serve as orientation markers.
Shipping
Ship on wet ice or dry ice
Product Usage Information
Application
Dilution
Load 5 μL per lane for standard mini-gel (0.75–1.0 mm thickness). For large-format gels, use 10–15 μL per lane. Load directly — no heating, reduction, or sample buffer addition required. Apparent molecular weights are determined under standard SDS-PAGE (Tris-Glycine) conditions and may vary slightly depending on gel chemistry and running conditions.
Stability and Storage
Store at -20°C upon receipt. Avoid repeated freeze-thaw cycles. Stable for up to 12 months when stored as directed.
Specifications
Molecular Weight Bands: 250, 130, 100, 70, 55, 35, 25, 15, 10 kDa (9 bands) Detection Methods: Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm) Stain Type: Tricolor (Blue, Orange, Green) Size Range: 10–250 kDa Gel Compatibility: Standard SDS-PAGE, Bis-Tris, Tris-Glycine, Tricine, and Tris-Acetate gel systems Format: Ready-to-use; no heating, reduction, or sample buffer needed Quantity: 2 × 250 μL/pack Applications: SDS-PAGE, Western Blot
_x005F_x000D_
Alternate Names
Prestained Protein Ladder, Tricolor Prestained Protein Marker, Pre-stained Protein Marker, Protein Molecular Weight Marker, MW Marker, 预染蛋白Marker
Background
Prestained protein markers are indispensable tools in protein electrophoresis and Western blotting workflows. By providing visible molecular weight references directly on the gel and membrane, they eliminate the need for post-run staining of the marker lane and enable real-time monitoring of electrophoretic separation and transfer efficiency. Tricolor markers with distinct chromatic bands further accelerate band identification, reducing the risk of misinterpreting molecular weight estimates. The 10–250 kDa range covers the vast majority of commonly studied proteins, making this marker suitable for routine laboratory use across diverse research applications.
中文
English
한국어
日本語
Español
Français
Русский
