Anti-PARP1 Polyclonal Antibody (HY482014)

Name: Anti-PARP1 Polyclonal Antibody
Brand: abinScience
SKU: HY482014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HY482014

Description

Anti-PARP1 Polyclonal Antibody (HY482014) is a rabbit polyclonal antibody detecting PARP1 in ELISA, IHC, WB. Suitable for Mouse, Bovine, Human, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Mouse, Bos taurus, Human, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PARP1 (Thr373-Glu540).

Target

PARP-1, Protein poly-ADP-ribosyltransferase PARP1, DNA ADP-ribosyltransferase PARP1, Poly [ADP-ribose] polymerase 1, PPOL, Poly[ADP-ribose] synthase 1, ADPRT 1, ADP-ribosyltransferase diphtheria toxin-like 1, ADPRT, PARP1, NAD(+) ADP-ribosyltransferase 1, ARTD1

Purification

Purified by antigen affinity column.

Accession

P09874

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Poly [ADP-ribose] polymerase 1 (PARP1) is a ~113 kDa protein. Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate, serine, histidine or tyrosine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Specificity for the different amino acids is conferred by interacting factors, such as HPF1 and NMNAT1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 confers serine specificity by completing the PARP1 active site.

1. Maruyama, T. et al. (2007) Clinical and experimental immunology 147, 164-75. PMID: 17177976

2. Langelier, MF. et al. (2008) The Journal of biological chemistry 283, 4105-14. PMID: 18055453

3. Ahel, I. et al. (2008) Nature 451, 81-5. PMID: 18172500

4. Reinemund, J. et al. (2009) Biochemical pharmacology 77, 1795-805. PMID: 19344625

5. Ahel, D. et al. (2009) Science (New York, N.Y.) 325, 1240-3. PMID: 19661379

6. Langelier, MF. et al. (2010) The Journal of biological chemistry 285, 18877-87. PMID: 20388712

7. Mao, Z. et al. (2011) Science (New York, N.Y.) 332, 1443-6. PMID: 21680843

8. Langelier, MF. et al. (2012) Science (New York, N.Y.) 336, 728-32. PMID: 22582261

9. Yan, Q. et al. (2013) Molecular and cellular biology 33, 845-57. PMID: 23230272

10. Vyas, S. et al. (2014) Nature communications 5, 4426. PMID: 25043379

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PARP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: 293T cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate