Anti-CD338/ABCG2 Polyclonal Antibody (HV314014)

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50ug
100ug

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Datasheet

Overview

Catalog No.

HV314014

Description

Anti-CD338/ABCG2 Polyclonal Antibody (HV314014) is a rabbit polyclonal antibody detecting ABCG2 in ELISA, IHC, WB. Suitable for Human and Cat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Cat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD338/ABCG2 (Asn557-His630).

Target

Urate exporter, ABCP, BCRP1, ABCG2, CD338, BCRP, MXR, Broad substrate specificity ATP-binding cassette transporter ABCG2, Placenta-specific ATP-binding cassette transporter, Breast cancer resistance protein, Mitoxantrone resistance-associated protein, CDw338, ATP-binding cassette sub-family G member 2

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q9UNQ0

RRID

Anti-CD338/ABCG2 Polyclonal Antibody (abinScience Cat# HV314014, RRID:AB_3730316)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Broad substrate specificity ATP-binding cassette transporter ABCG2 (CD338) is a ~72 kDa protein. Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme. Also mediates the efflux of sphingosine-1-P from cells. Acts as a urate exporter functioning in both renal and extrarenal urate excretion. In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate.

1. Komatani, H. et al. (2001) Cancer research 61, 2827-32. PMID: 11306452

2. Zhang, W. et al. (2003) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 17, 2085-7. PMID: 12958161

3. Woodward, OM. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 10338-42. PMID: 19506252

4. Desuzinges-Mandon, E. et al. (2010) The Journal of biological chemistry 285, 33123-33133. PMID: 20705604

5. Taylor, NMI. et al. (2017) Nature 546, 504-509. PMID: 28554189

6. Manolaridis, I. et al. (2018) Nature 563, 426-430. PMID: 30405239

7. Toyoda, Y. et al. (2019) Cells 8. PMID: 31003562

8. Kobuchi, H. et al. (2012) PloS one 7, e50082. PMID: 23189181

9. Takabe, K. et al. (2010) The Journal of biological chemistry 285, 10477-86. PMID: 20110355

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD338/ABCG2 polyclonal antibody at 1ug/mL on recombinant CD338/ABCG2 (Catalog No. HV314012).

References

Datasheet

Anti-CD338/ABCG2 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CD338/ABCG2 Polyclonal Antibody (HV314014)

Overview

Images

References

Datasheet

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