Anti-SARS-CoV-2 ORF3a Polyclonal Antibody (VK577014)

Name: Anti-SARS-CoV-2 ORF3a Polyclonal Antibody
Brand: abinScience
SKU: VK577014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

VK577014

Description

Anti-SARS-CoV-2 ORF3a Polyclonal Antibody (VK577014) is a rabbit polyclonal antibody detecting ORF3a protein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Severe acute respiratory syndrome coronavirus 2

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant SARS-CoV-2 ORF3a (Leu115-Leu275).

Target

ORF3a protein, ORF3a, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, 3a

Purification

Purified by antigen affinity column.

Accession

P0DTC3

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

ORF3a protein is a ~31 kDa protein. Plays a role in viral egress via lysosomal trafficking. Forms homotetrameric ion channels (viroporins) localized at endosomes and lysosomes, that may induce deacidification of lysosomes, allowing safe egress of virions via lysosomal trafficking. Also blocks autolysosome formation by binding and sequestering the host component VPS39 for homotypic fusion and protein sorting (HOPS) on late endosomes. This prevents fusion of autophagosomes with lysosomes, disrupting autophagy and facilitating virus egress. Induces host RETREG1/FAM134B-dependent reticulophagy by interacting with host HMGB1 and enhancing the association between HMGB1 and host BECN1.

1. Ghosh, S. et al. (2020) Cell 183, 1520-1535.e14. PMID: 33157038

2. Miao, G. et al. (2021) Developmental cell 56, 427-442.e5. PMID: 33422265

3. Kern, DM. et al. (2021) Nature structural & molecular biology 28, 573-582. PMID: 34158638

4. Zhang, X. et al. (2022) Autophagy 18, 2576-2592. PMID: 35239449

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SARS-CoV-2 ORF3a polyclonal antibody at 1ug/mL on recombinant SARS-CoV-2 ORF3a (Catalog No. VK577012).

References

Datasheet

Anti-SARS-CoV-2 ORF3a Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-SARS-CoV-2 ORF3a Polyclonal Antibody (VK577014)

Overview

Images

References

Datasheet

SDS

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