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Anti-Rat SCNN1A/SCNN1 Polyclonal Antibody (RW302014)

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Overview
Catalog No.RW302014
Description
Anti-Rat SCNN1A/SCNN1 Polyclonal Antibody (RW302014) is a rabbit polyclonal antibody detecting Scnn1a in ELISA, IHC, WB. Suitable for Human, Mouse, Pan troglodytes, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityMouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Rat SCNN1A/SCNN1 (Glu132-Asp391).
Target Amiloride-sensitive sodium channel subunit alpha, Alpha-NaCH, Epithelial Na(+) channel subunit alpha, Alpha-ENaC, Nonvoltage-gated sodium channel 1 subunit alpha, SCNEA, Scnn1a, Renac
Purification Purified by antigen affinity column.
Accession P37089
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Epithelial sodium channel subunit alpha (SCNN1A/SCNN1) is a ~78 kDa protein. This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis. ENaC operates in epithelial tissues, where it mediates the electrodiffusion of sodium ions from extracellular fluid through the apical membrane of cells, with water following osmotically. It plays a key role in maintaining sodium homeostasis through electrogenic sodium reabsorption in the kidneys. Additionally, ENaC is essential for airway surface liquid homeostasis, which is crucial for proper mucus clearance.

1. Lingueglia, E. et al. (1994) The Journal of biological chemistry 269, 13736-9. PMID: 8188647
2. Gründer, S. et al. (1997) The EMBO journal 16, 899-907. PMID: 9118951
3. Lingueglia, E. et al. (1993) FEBS letters 318, 95-9. PMID: 8382172
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Rat SCNN1A/SCNN1 Polyclonal Antibody

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