Anti-Human APEX1 Polyclonal Antibody (HB917014)

Name: Anti-Human APEX1 Polyclonal Antibody
Brand: abinScience
SKU: HB917014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HB917014

Description

Anti-Human APEX1 Polyclonal Antibody (HB917014) is a rabbit polyclonal antibody detecting APEX1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pan paniscus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human APEX1 (Thr61-Leu318).

Target

APX, APE1, APEX nuclease, APEN, Redox factor-1, APE-1, REF1, APEX, APE, Apurinic-apyrimidinic endonuclease 1, HAP1, AP endonuclease 1, APEX1, DNA-(apurinic or apyrimidinic site) endonuclease, REF-1

Purification

Purified by antigen affinity column.

Accession

P27695

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA repair nuclease/redox regulator APEX1 is a ~35 kDa protein. Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are DNA repair and redox regulation of transcriptional factors. Functions as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Also incises at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules.

1. Wei, SJ. et al. (2000) Cancer research 60, 6688-95. PMID: 11118054

2. Hsieh, MM. et al. (2001) Nucleic acids research 29, 3116-22. PMID: 11452037

3. Parsons, JL. et al. (2005) Nucleic acids research 33, 2204-9. PMID: 15831793

4. Berquist, BR. et al. (2008) Journal of molecular biology 379, 17-27. PMID: 18439621

5. Georgiadis, MM. et al. (2008) Mutation research 643, 54-63. PMID: 18579163

6. Kim, WC. et al. (2011) Journal of molecular biology 411, 960-71. PMID: 21762700

7. Schermerhorn, KM. et al. (2013) Biochemistry 52, 7669-77. PMID: 24079850

8. Walker, LJ. et al. (1993) Molecular and cellular biology 13, 5370-6. PMID: 8355688

9. Hirota, K. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 3633-8. PMID: 9108029

10. Ramana, CV. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 5061-6. PMID: 9560228

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-APEX1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Raji cell lysate
Lane 3: 293T cell lysate
Lane 4: Mouse spleen lysate

References

Datasheet

Anti-Human APEX1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human APEX1 Polyclonal Antibody (HB917014)

Overview

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References

Datasheet

SDS

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