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Anti-Human CYP2C8 Polyclonal Antibody (HY115014)

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Overview
Catalog No.HY115014
Description
Anti-Human CYP2C8 Polyclonal Antibody (HY115014) is a rabbit polyclonal antibody detecting CYP2C8 in ELISA, IHC, WB. Suitable for Human and Macaca fascicularis.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CYP2C8 (Gly40-Val490).
Target CYP2C8, Cytochrome P450 MP-12, Cytochrome P450 IIC2, Cytochrome P450 MP-20, Cytochrome P450 2C8, CYPIIC8, S-mephenytoin 4-hydroxylase, Cytochrome P450 form 1
Purification Purified by antigen affinity column.
Accession P10632
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Cytochrome P450 2C8 (CYP2C8) is a ~55 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Primarily catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA) with a preference for the last double bond. Catalyzes the hydroxylation of carbon-hydrogen bonds. Metabolizes all trans-retinoic acid toward its 4-hydroxylated form.

1. Marill, J. et al. (2000) Molecular pharmacology 58, 1341-8. PMID: 11093772
2. Lee, AJ. et al. (2003) Cancer research 63, 6532-6. PMID: 14559847
3. Barbosa-Sicard, E. et al. (2005) Biochemical and biophysical research communications 329, 1275-81. PMID: 15766564
4. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576
5. Zeldin, DC. et al. (1995) Archives of biochemistry and biophysics 322, 76-86. PMID: 7574697
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CYP2C8 Polyclonal Antibody

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